psiNAT HLA B27 Real Time PCR Test kit for the qualitative detection of nucleic acid from peripheral blood samples of suspected individuals. Human leukocyte antigen (HLA) (subtypes B) is a class I surface antigen encoded by the B locus in the major histocompatibility complex (MHC) on chromosome 6 and presents antigenic peptides (derived from self and non-self-antigens) to T cells. HLA-B27 is strongly associated with ankylosing spondylitis and other associated inflammatory diseases, such as psoriatic arthritis, inflammatory bowel disease, and reactive arthritis. psiNAT HLA B27 Real Time PCR Test kit targets the specific conserved sequence encoding the HLA-B gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.