Introduction

psiNAT Adenovirus Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Adenoviruses (family Adenoviridae) are medium-sized (90–100 nm), nonenveloped viruses with an icosahedral nucleocapsid containing a double-stranded DNA genome. Some strains of adenovirus will cause infections in the stomach and gastrointestinal tract. This can lead to diarrhea, abdominal cramps, and other symptoms of gastroenteritis. psiNAT Adenovirus Real Time PCR Test kit targets the specific conserved sequence encoding the hexon protein gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Campylobacter Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Campylobacter is a genus of Gram-negative bacteria. Campylobacter, most commonly Campylobacter jejuni, cause stomach flu (gastroenteritis). The most common symptoms are diarrhea, vomiting, stomach cramping and fever. psiNAT Campylobacter Real Time PCR Test kit targets the specific conserved sequence encoding the 16S rRNA gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Clostridium Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Clostridium difficile, also known as C. difficile is Gram-positive species of spore-forming bacteria. Clostridium difficile infection (CDI) is due to a toxin-producing bacteria that causes a more severe form of antibiotic associated diarrhea. The disease ranges from mild diarrhea to severe colon inflammation that can even be fatal. CDI usually occurs when people have taken antibiotics that change the normal colon bacteria allowing the C. difficile bacteria to grow and produce its toxins. psiNAT Clostridium Real Time PCR Test kit targets the specific conserved sequence encoding the tcdA & tcdB gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Cryptosporidium Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Cryptosporidium is a genus of apicomplexan parasitic alveolates that can cause a respiratory and gastrointestinal illness (cryptosporidiosis) that primarily involves watery diarrhea (intestinal cryptosporidiosis) with or without a persistent cough (respiratory cryptosporidiosis) in both immunocompetent and immunodeficient humans. psiNAT Cryptospordium Real Time PCR Test kit targets the specific conserved sequence encoding the DNAJ-like protein gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT E.coli Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Escherichia coli also known as E.coli is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms. Most types of E. coli are harmless or cause relatively brief diarrhea. But a few strains, such as E. coli O157:H7, can cause severe stomach cramps, bloody diarrhea and vomiting. E. coli O157:H7 produces a toxin known as Shiga. It is one of the most powerful toxins, and it can cause an intestinal infection. psiNAT E.coli Real Time PCR Test kit targets the specific conserved sequence encoding the lacY gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNATEntamoeba Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Entamoeba histolytica is an anaerobic parasitic amoebozoan, part of the genus Entamoeba.Amebiasis is the infection of the human gastrointestinal tract by Entamoeba histolytica, a protozoan parasite that is capable of invading the intestinal mucosa and may spread to other organs, mainly the liver.psiNATEntamoeba Real Time PCR Test kit targets the specific conserved sequence encoding the 18S rRNA gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Giardia Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Giardia is a genus of anaerobic flagellated protozoan parasites of the phylum Metamonada that colonise and reproduce in the small intestines of several vertebrates, causing the disease giardiasis. It is an intestinal infection marked by stomach cramps, bloating, nausea and bouts of watery diarrhea. It is mainly caused by Giardia duodenalis (also known as G. lamblia and G. intestinalis). Giardia is found on surfaces or in soil, food, or water that has been contaminated with feces from infected people or animals. psiNAT Giardia Real Time PCR Test kit targets the specific conserved sequence encoding the 18S rRNA gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Gastrointestinal Multiplex Real Time RT- PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. There are a number of bacteria, viruses, and parasites that cause gastrointestinal (GI) infections. Bacterial gastrointestinal infections include foodborne infections and food poisoning.Viral gastrointestinal infections are very common, and often people refer to these as the stomach flu. Intestinal helminths, or worms, and protozoan parasites cause parasitic gastrointestinal infections.psiNAT Gastrointestinal Multiplex Real Time RT- PCR Test kit is for the detection of viruses like Adenovirus, Rotavirus, Norovirus bacteria like Camphylobacter, Clostridium difficile, E.coli, Salmonella, Shigella, Yersinia and parasites like Cryptosporidium, Giardia, Entamoeba, and targets the specific conserved sequence encoding the hexon protein gene of Adenovirus, 16S rRNA gene of Campylobacter, tcdA&tcdBgene of Clostridium difficile, DNAJ-like protein gene of Cryptosporodium, lacY gene of E.coli, 18S rRNA gene of Giardia, 18S rRNA gene of Entamoeba histolytica, NSP3 gene of Rotavirus, ttrC gene of Salmonella, ipaH gene of Shigella,foxA gene of Yersinia enterocolitica, ORF1 gene of Norovirus. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Rotavirus Real Time RT-PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Rotavirus is a genus of double-stranded RNA viruses in the family Reoviridae. Rotavirus is a very contagious virus that causes inflammation in the stomach and intestines. It can cause severe diarrhea (Rotavirus gastroenteritis), vomiting, fever, belly pain, and dehydration in infants, young children, and some adults. psiNAT Rotavirus RT-Real Time PCR Test kit targets the specific conserved sequence encoding the NSP3 gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNATSalmonella Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Salmonella is a genus of rod-shaped (bacillus) Gram-negative bacteria of the family Enterobacteriaceae.Salmonella infection (salmonellosis) is a common bacterial disease that affects the intestinal tract. Salmonella bacteria typically live in animal and human intestines and are shed through stool (feces). Humans become infected most frequently through contaminated water or food.psiNAT Salmonella Real Time PCR Test kit targets the specific conserved sequence encoding the ttrCgene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Shigella Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Shigella is a genus of bacteria that is Gram-negative, facultative anaerobic, non-spore-forming, nonmotile and rod-shaped. Shigella bacteria cause an infection called shigellosis that causes diarrhea (sometimes bloody), fever, and stomach cramps. psiNAT Shigella Real Time PCR Test kit targets the specific conserved sequence encoding the ipaH gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Vibrio Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Vibrio cholerae is a species of Gram-negative, facultative anaerobe and comma-shaped bacteria that causes the diarrheal disease cholera. Cholera is an acute, diarrheal illness caused by infection of the intestine with the toxigenic bacterium Vibrio cholerae serogroup O1 or O139. Cholera can be life-threatening but it is easily prevented and treated. psiNAT Vibrio Real Time PCR Test kit targets the specific conserved sequence encoding the ompW gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

Introduction

psiNAT Yersinia Real Time PCR Test kit for the qualitative detection of nucleic acid from stool sample of suspected patients. Yersinia is a genus of bacteria in the family Yersiniaceae. Yersinia species are Gram-negative, coccobacilli bacteria and are facultative anaerobes. Yersiniosis is an infection caused most often by eating raw or undercooked pork contaminated with Yersinia enterocolitica bacteria. The infection is manifested as acute diarrhea, mesenteric adenitis, terminal ileitis, and pseudoappendicitis. psiNAT Yersinia Real Time PCR Test kit targets the specific conserved sequence encoding the foxA gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real-time PCR instrument. The PCR detection system includes an endogenous internal control too.

• Pathogen: Norovirus
• Transmission: Contaminated food, water, surfaces
• Symptoms: Acute gastroenteritis, diarrhea, vomiting

• Pathogen: Hepatitis A Virus
• Transmission: Fecal-oral route, often through contaminated food or water
• Symptoms: Fatigue, nausea, abdominal pain, jaundice

• Pathogen: Clostridium perfringens
• Transmission: Contaminated food (especially meats)
• Symptoms: Food poisoning, diarrhea, abdominal cramps